Anti-GDI1 Polyclonal Antibody for WB and ELISA - P31150

Core Product Specifications and Parameters

Biological Background: GDI1 Function and Localization

• GDI1, or Rab GDP dissociation inhibitor alpha, is also known as Guanosine diphosphate dissociation inhibitor 1 (GDI-1), Oligophrenin-2, and Protein XAP-4.
• It regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them and the subsequent binding of GTP.
• GDI1 promotes the dissociation of GDP-bound Rab proteins from membranes and inhibits their activation; specific Rab targets include RAB1A, RAB3A, RAB5A, and RAB10.
• The protein localizes to the cytoplasm, Golgi apparatus, and trans-Golgi network.
• GDI1 is predominantly expressed in the brain and neural/sensory tissues.
• Mutations in GDI1 are associated with intellectual disability, highlighting its critical role in neuronal function.
• Additional keywords include GTPase activation, direct protein sequencing, proteomics identification, and it is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein covering amino acids 188–447 of human GDI1; this region spans the C-terminal portion, which may confer specificity.
• For Western blot (WB), consider using brain tissue lysates or cell lines expressing endogenous GDI1, as the protein is enriched in neural tissues.
• When performing ELISA, ensure optimal coating conditions and validate with appropriate positive and negative controls.
• Cross-reactivity with human and mouse is reported; verify performance in your species of interest if using other models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with efficient access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For studies on Rab protein regulation and neurological disorders, we offer reliable sourcing of rare target raw materials such as GDI1 antibodies. Our integrated supply chain streamlines procurement from concept to clinic, supported by technical consulting services.