Anti-GDF9 Polyclonal Antibody for WB, ELISA - O60383

Core Product Specifications and Parameters

Biological Background: GDF9 Function and Localization

• GDF9 (Growth/differentiation factor 9), also known as POF14, is a secreted growth factor critical for ovarian folliculogenesis.
• It is required for primordial follicle development and stimulates granulosa cell proliferation.
• GDF9 promotes cell cycle progression from G0/G1 to S and G2/M phases by upregulating CCND1 and CCNE1 expression and inducing RB1 phosphorylation.
• It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells, playing a key role in steroidogenesis.
• GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing inhibin B expression, and suppresses FST and FSTL3 production in granulosa-lutein cells.
• Subcellularly, GDF9 is secreted; it is expressed in ovarian granulosa cells and present in oocytes of primary follicles.
• UniProt keywords highlight features such as cleavage on pair of basic residues, disulfide bond, glycoprotein, and phosphoprotein; it is also associated with premature ovarian failure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning the mature domain of human GDF9 (amino acids 205-454), which may provide specific recognition of the processed active form.
• For Western blot, the predicted molecular weight is 51 kDa; consider using appropriate reducing conditions and validate the observed band in your sample system.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies in rodent models; always confirm reactivity in your specific context.
• Optimize dilution factors for your particular sample type and detection system, as performance may vary with experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for reproductive health research. This GDF9 antibody supports ovarian biomarker development, and our team can assist with rare target raw-material sourcing to streamline your diagnostic assay production from concept to clinic.