Anti-GDF15 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q99988

Core Product Specifications and Parameters

Biological Background: GDF15 Function and Localization

• GDF15 is a divergent member of the TGF-β superfamily, also known as MIC-1, NAG-1, PLAB, and PTGFB, functioning as a secreted cytokine and hormone.
• As a stress-responsive hormone, GDF15 binds to the GFRAL receptor in the brainstem, triggering nausea, vomiting, and appetite suppression as part of an aversive response to minimize exposure to damaging agents. Related references: PMID:23468844 PMID:24971956 PMID:28846097 PMID:28846098 PMID:28846099 PMID:28953886 PMID:30639358 PMID:33589633 PMID:38092039
• GDF15 contributes to metformin-induced weight loss by being produced in the kidney and activating the GDF15-GFRAL axis, though its contribution is debated. Related references: PMID:31875646 PMID:37060902 PMID:36001956
• Overproduction of GDF15 in cancer and during chemotherapy promotes anorexia and cachexia. Related references: PMID:32661391
• During pregnancy, high fetal-derived GDF15 levels are associated with increased nausea and vomiting, with maternal sensitivity influenced by pre-pregnancy GDF15 levels. Related references: PMID:38092039
• GDF15 promotes metabolic adaptation and tissue tolerance during systemic inflammation, including protection against cardiac rupture after myocardial infarction. Related references: PMID:31402172
• GDF15 is highly expressed in the placenta, with lower levels in prostate, colon, and kidney, and is detectable in plasma. Related references: PMID:28572090 PMID:29046435 PMID:37060902 PMID:9348093
• The protein is synthesized as a precursor, cleaved on basic residues to generate the mature form, and is secreted as a disulfide-linked homodimer with an apparent molecular weight of ~34 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 49-308 of mature human GDF15; consider epitope exposure when optimizing for WB under reducing or non-reducing conditions.
• For IHC-P and IF/ICC applications, validate with appropriate antigen retrieval methods and positive tissue controls such as placenta or cancer specimens.
• In ELISA, this polyclonal antibody can be paired with a monoclonal capture antibody for sandwich format; perform checkerboard titrations to determine optimal coating and detection concentrations.
• When testing cross-reactivity in mouse or rat samples, use corresponding tissue lysates or recombinant proteins to confirm specificity and band size.
• For IP, confirm antibody-protein complex formation with a protein A/G-compatible buffer system and note the expected molecular weight shift.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive selection of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. For researchers focused on GDF15 in cachexia, metabolic signaling, or pregnancy-related disorders, we offer tailored sourcing solutions—from this anti-GDF15 polyclonal antibody to complementary matched pairs and rare-target reagents. Let us bridge your journey from concept to clinic with reliable, one-stop supply and technical support.