Anti-GDF15 Monoclonal Antibody for WB and ELISA - Q99988

Core Product Specifications and Parameters

Biological Background: GDF15 Function and Localization

• GDF15, also known as MIC-1, NAG-1, PLAB, PTGFB, and PDF, is a secreted member of the TGF-beta superfamily with hormone and cytokine activities.
• Acts as a hormone produced in response to various stresses to trigger an aversive response (nausea, vomiting, loss of appetite) via brain circuits, promoting avoidance behavior. Related references: PMID:23468844, PMID:24971956, PMID:28846097, PMID:30639358, PMID:33589633, PMID:38092039
• Binds selectively to its receptor GFRAL on neurons in the brainstem (area postrema and nucleus tractus solitarius), activating downstream emergency circuits. Related references: PMID:28846097, PMID:28846098, PMID:28846099, PMID:28953886
• Suppresses appetite and promotes weight loss; contributes to the anorectic effects of metformin, though the quantitative contribution is debated. Related references: PMID:31875646, PMID:37060902, PMID:36001956
• Overproduced in many cancers and drives cancer cachexia; anticancer drugs such as camptothecin or cisplatin induce GDF15, leading to nausea and malnutrition. Related references: PMID:32661391
• High fetal-origin GDF15 levels during pregnancy are associated with nausea and vomiting; maternal sensitivity depends on pre-pregnancy GDF15 exposure. Related references: PMID:38092039
• Promotes metabolic adaptation and tissue tolerance during systemic inflammation by modulating lipid metabolism and inhibiting leukocyte integrin activation. Related references: PMID:31402172
• Subcellular location: Secreted. Highly expressed in placenta, with lower levels in prostate, colon, and kidney. Related references: PMID:9348093, PMID:28572090, PMID:29046435, PMID:37060902

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the propeptide region (amino acids 100-200); therefore, the antibody is expected to detect the full-length precursor (~34 kDa) on Western blot. The mature secreted form (~12.5 kDa) may not be recognized if the epitope is removed during processing.
• For Western blot, use reducing conditions and load positive control lysates from GDF15-expressing tissues (e.g., placenta, prostate, colon) or cell lines. Optimal antibody dilution should be determined empirically.
• For ELISA, the antibody can be evaluated as a capture or detection reagent; validate pairing with a different anti-GDF15 clone for sandwich assays. Serum or plasma samples are appropriate given the secreted nature of the target.
• Keep samples cold and avoid repeated freeze-thaw cycles to preserve antigen integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop partner for IVD raw-material supply, covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for critical research areas such as metabolic signaling, cancer biology, and pregnancy-related disorders. For your GDF15 assay development, we can source rare targets and provide technical consulting to accelerate your project.