Anti-GDF-8/Myostatin Rabbit Monoclonal Antibody for WB and ELISA - O14793

Core Product Specifications and Parameters

Biological Background: GDF-8/Myostatin Function and Localization

• Myostatin (GDF-8) functions as a specific negative regulator of skeletal muscle growth.
• It is a secreted protein, as indicated by its subcellular localization and the presence of a signal peptide.
• The protein is synthesized as a precursor and undergoes proteolytic cleavage on a pair of basic residues to generate the mature, active form.
• Post-translational modifications include glycosylation and the formation of intramolecular disulfide bonds, which are critical for its structure and function.
• Myostatin can bind to heparin, a property that may influence its distribution and activity in the extracellular matrix.
• The protein has been structurally characterized by 3D-structure determination, providing insights into its receptor interactions and regulation.
• It is a reference proteome member, indicating its well-characterized status in human proteomics.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 24–375, covering both the propeptide and mature domains; consider this when interpreting band patterns in WB, as both precursor and cleaved forms may be detected.
• For ELISA applications, consider direct or sandwich formats given the antibody's recognition of myostatin in human, mouse, and rat.
• Use appropriate anti-rabbit secondary antibodies for detection in immunoassays.
• Validate the antibody in your specific sample matrix and experimental conditions, as performance may vary with sample preparation and denaturation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted sourcing partner for GDF-8/myostatin research and assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your workflow. Our team is ready to assist with rare target raw-material sourcing and customization, ensuring you have the tools needed for reliable IVD applications.