Anti-GDF-8/Myostatin Polyclonal Antibody for WB, IHC-P, ELISA - O14793

Core Product Specifications and Parameters

Biological Background: GDF-8/Myostatin Function and Localization

• GDF-8, also known as myostatin, is a member of the transforming growth factor-beta (TGF-β) superfamily of growth and differentiation factors.
• It functions as a negative regulator of skeletal muscle growth, limiting muscle mass.
• The protein is secreted and acts as a cytokine/growth factor, binding to activin type II receptors and signaling through SMAD pathways.
• Subcellular localization is primarily extracellular after secretion; it is synthesized as a precursor and proteolytically cleaved to generate the active mature peptide.
• Keywords include growth factor, heparin-binding, disulfide bond, glycoprotein, and reference proteome, indicating post-translational modifications and structural features.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 267–375) of human myostatin, which includes part of the mature active domain; this design may favor detection of the precursor and mature forms.
• For Western blot, a band around 43 kDa (precursor) and possibly a smaller band (~12.5 kDa mature peptide) may be observed; consider using reducing conditions.
• This antibody is indicated for WB, IHC-P, and ELISA; validate dilutions in the relevant sample system, as reactivity may vary across different tissues and species.
• Cross-reactivity with mouse and rat myostatin is expected due to high sequence homology; however, confirm performance if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to IVD raw materials, from validated antibodies to bulk ancillary reagents. This anti-GDF-8/Myostatin polyclonal antibody supports muscle growth and differentiation research, and we can source custom formulations or matched antibody pairs to accelerate your development. Let CamelBio be your sourcing partner for rare targets and complete IVD reagent kits.