Anti-GDAP1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8TB36

Core Product Specifications and Parameters

Biological Background: GDAP1 Function and Localization

• GDAP1 is a mitochondrial outer membrane protein that promotes mitochondrial fission, playing a key role in the regulation of mitochondrial morphology and dynamics.
• It is highly expressed in the central nervous system, particularly in the brain and spinal cord, and is found in both neurons and Schwann cells.
• Subcellularly, GDAP1 localizes to the mitochondrial outer membrane and the cytoplasm.
• Mutations in GDAP1 are causatively linked to autosomal recessive Charcot-Marie-Tooth disease type 4A (CMT4A), a severe peripheral neuropathy characterized by demyelination and axonal loss.
• The protein contains a transmembrane domain and a coiled-coil region, and undergoes post-translational modifications including acetylation and ubiquitination.
• Alternative splicing generates multiple isoforms, which may have distinct functions in mitochondrial dynamics and intracellular signaling.

Experimental Guidance and Technical Tips

• Based on the immunogen design targeting the N-terminal aa 1-225 of human GDAP1, the antibody is predicted to recognize multiple isoforms, including those resulting from alternative splicing.
• For Western blot (WB), an expected band size of approximately 41 kDa (full-length protein) may be observed; additional bands could correspond to processed or modified forms.
• In immunohistochemistry (IHC-P), tissues with high GDAP1 expression, such as brain and spinal cord from human, mouse, or rat, may serve as appropriate positive controls.
• For ELISA applications, consider using the recombinant immunogen as a standard to establish assay conditions and verify antibody specificity.
• Due to the conservation of GDAP1 across human, mouse, and rat, cross-species reactivity is expected, but individual experiments should be validated under the user’s specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering antibodies like this anti-GDAP1 polyclonal antibody to support mitochondrial and neurodegenerative disease research. Our extensive inventory includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, streamlining your transition from early-stage development to clinical application. With a focus on rare and specialized targets, CamelBio ensures reliable supply and technical support for your diagnostic manufacturing needs.