Anti-GCSH Rabbit Monoclonal Antibody (WB, IF/ICC, ELISA) - P23434

Core Product Specifications and Parameters

Biological Background: GCSH Function and Localization

• The GCSH gene encodes the glycine cleavage system H protein, a mitochondrial lipoic acid-containing protein also known as lipoic acid-containing protein.
• GCSH is a critical component of the glycine cleavage system, where it shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST) for degradation.
• Beyond glycine metabolism, GCSH plays a pivotal role in the lipoylation of mitochondrial enzymes such as DLAT (pyruvate dehydrogenase complex) and DLST (2-oxoglutarate dehydrogenase complex), impacting cellular energetics. Related references: PMID:36190515
• The protein localizes to the mitochondrion, consistent with its transit peptide and mitochondrial keyword annotations.
• UniProt keywords include Disease variant, Neurodegeneration, Lipoyl, and 3D-structure, highlighting its clinical relevance and structural characterization.
• The mature protein has a calculated molecular weight of approximately 18.9 kDa (UniProt), with an observed band around 19 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), an expected band at ~19 kDa is observed; consider using mitochondrial-enriched lysates for improved detection.
• In immunofluorescence (IF/ICC), mitochondrial staining typically requires permeabilization; validate staining specificity with appropriate controls.
• The immunogen (aa 49-173) excludes the transit peptide, targeting the mature protein; cross-reactivity with other lipoic acid-containing proteins is unlikely but should be verified.
• For ELISA, standard sandwich or indirect formats may be applied; assay conditions should be optimized for the specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive IVD raw-material sourcing, technical services, and consulting from concept to clinical application. This anti-GCSH monoclonal antibody serves as a reliable tool for studying glycine metabolism, mitochondrial disorders, and neurodegeneration. Explore our portfolio for validated antibody pairs, bulk ancillary reagents, and custom monoclonal/polyclonal antibody development to streamline your diagnostic and research workflows.