Anti-GCN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P2K8

Core Product Specifications and Parameters

Biological Background: GCN2 Function and Localization

• GCN2, encoded by EIF2AK4, is a metabolic-stress sensing serine/threonine-protein kinase that phosphorylates eIF-2-alpha in response to low amino acid availability, activating the integrated stress response (ISR). Related references: PMID:25329545 PMID:32610081
• Phosphorylation of eIF-2-alpha converts it into a global translation inhibitor, attenuating cap-dependent protein synthesis while enabling selective translation of ISR-specific mRNAs such as ATF4, thereby reprogramming amino acid biosynthetic gene expression. Related references: PMID:32610081
• The protein is involved in cell cycle regulation by promoting translation repression of cyclin D1 mRNA upon UPR activation or translation activation of CDKN1A/p21 under amino acid deprivation. Related references: PMID:26102367
• GCN2 contains an ATP-binding domain, a kinase domain, and a tRNA-binding domain, allowing it to directly sense uncharged tRNAs and link nutrient status to translational control.
• It localizes primarily to the cytoplasm and is widely expressed, with notable presence in lung, smooth muscle cells, and macrophages.
• The kinase plays a pro-survival and proapoptotic role depending on stress context, and is also involved in antiviral defense, promoting autophagy and antigen presentation during yellow fever virus infection. Related references: PMID:24310610
• Alternative splicing generates multiple isoforms, and the protein is subject to post-translational modifications including acetylation and phosphorylation, which regulate its activity.
• Beyond nutrient sensing, GCN2 contributes to synaptic plasticity, learning, long-term memory formation, and neurite outgrowth inhibition in neuronal contexts.

Experimental Guidance and Technical Tips

• For WB, consider using a low-percentage (e.g., 6–8%) SDS-PAGE gel to resolve the high molecular weight GCN2 protein (~187 kDa) and optimize transfer conditions for large proteins.
• In IF/ICC, expect predominantly cytoplasmic staining; validate subcellular localization with organelle markers in your cell system of interest.
• The immunogen corresponds to the N-terminal region (aa 1–300); verify specificity in the relevant species (human, mouse, rat) using positive and negative controls.
• Pair with appropriate loading controls and consider using protease/phosphatase inhibitors during lysis due to potential post-translational modifications affecting protein migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material sourcing partner, streamlining procurement of validated GCN2 antibodies and ancillary reagents for integrated stress response research. Beyond this polyclonal antibody, we offer one-stop access to monoclonal antibodies, optimized antibody pairs, bulk formulation-grade reagents, and custom sourcing for rare target raw materials—supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic.