Anti-GCN1L1 (GCN1) Rabbit Polyclonal Antibody for WB and ELISA - Q92616

Core Product Specifications and Parameters

Biological Background: GCN1L1 (GCN1) Function and Localization

• GCN1L1, also known as GCN1, GCN1-like protein 1, or translational activator GCN1, is a large ~293 kDa protein that functions as a stalled ribosome sensor.
• It directly binds to ribosomes and acts as a sentinel for colliding ribosomes. Upon ribosome stalling, GCN1 promotes recruitment of the E3 ubiquitin ligase RNF14, which ubiquitinates EEF1A1/eEF1A and other translation factors, targeting them for degradation. Related references: PMID:32610081, PMID:36638793, PMID:37651229, PMID:37951215, PMID:37951216
• GCN1 also mediates degradation of ETF1/eRF1 and ubiquitination of ribosomal proteins via the RNF14-RNF25 pathway, ensuring clearance of stalled translation complexes. Related references: PMID:36638793, PMID:37651229
• As a positive activator of the integrated stress response (ISR), GCN1 interacts with the kinase EIF2AK4/GCN2 on translating ribosomes, stimulating its activity. This leads to phosphorylation of eIF-2-alpha and global translation attenuation, while allowing selective translation of stress-responsive mRNAs such as ATF4.
• GCN1 is primarily localized in the cytoplasm, where it monitors translating ribosomes.
• It is ubiquitously expressed, with notable expression in skeletal muscle, ovary, and testis. Related references: PMID:9039502, PMID:9234705
• The protein undergoes acetylation and phosphorylation, and features coiled-coil domains and repeats, consistent with its large scaffolding role.
• Keywords from UniProt also include Stress response, Translation regulation, Activator, highlighting its central role in cellular proteostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 2462–2671), which may provide specificity for full-length GCN1L1. Consider verifying antibody reactivity using lysates from human cell lines with known GCN1L1 expression.
• For Western blotting, the predicted molecular weight is ~293 kDa; use appropriate gel percentage and transfer conditions for high molecular weight proteins.
• ELISA can be used to detect and quantify GCN1L1 in human samples; consider pairing with a matched detection antibody for sandwich ELISA.
• Since GCN1L1 is ubiquitously expressed, it can serve as a loading control for high molecular weight proteins; however, validate expression stability across experimental conditions.
• Cross-reactivity is reported only with human; for other species, perform sequence alignment to assess potential reactivity.
• Shipping on ice bags suggests the antibody is stable under cold chain; store at recommended temperature upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports translation research and proteostasis studies with fully characterized antibodies like this anti-GCN1L1 polyclonal antibody. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your diagnostic and research workflows. Contact us to discuss your specific requirements.