Anti-GCM1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NP62

Core Product Specifications and Parameters

Biological Background: GCM1 Function and Localization

• GCM1 (Chorion-specific transcription factor GCMa), also known as GCMA or hGCMa, is a zinc-containing transcription factor that controls expression of placental growth factor (PGF) and other placenta-specific genes. Related references: PMID:10542267 PMID:18160678
• Binds to the trophoblast-specific element 2 (TSE2) of the aromatase gene enhancer. Related references: PMID:10542267
• Binds to the SYDE1 promoter. Related references: PMID:27917469
• Plays a central role in mediating the differentiation of trophoblast cells along both the villous and extravillous pathways in placental development. Related references: PMID:19219068
• Localizes to the nucleus, consistent with its role as a transcription factor.
• Highly expressed in the placenta, particularly in trophoblast cells of the villi.
• Associated keywords: developmental protein, DNA-binding, metal-binding, transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 207-436 of human GCM1; epitope specificity within this region has not been mapped.
• For Western blot, a signal near 49 kDa may be observed in placental tissue lysates; include positive and negative controls.
• In immunofluorescence/ICC, expect nuclear staining; validate with appropriate organelle markers.
• For ELISA, consider coating with recombinant GCM1 fragment (aa 207-436) as a positive control and optimize antibody dilution.
• As a polyclonal antibody, lot-to-lot variation is possible; validate each new lot for consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing provider for IVD raw materials, offering validated antibodies, antigens, and ancillary reagents for placental and developmental biology research. For GCM1-related studies, we supply this polyclonal antibody alongside optimized monoclonal antibodies, antigen pairs, and bulk ancillary reagents to support your assay development needs. Our capabilities extend to rare target sourcing, ensuring reliable materials for trophoblast differentiation and pregnancy-related disorder research.