Anti-GCLM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P48507

Core Product Specifications and Parameters

Biological Background: GCLM Function and Localization

• GCLM (Glutamate--cysteine ligase regulatory subunit) is the modifier subunit of glutamate-cysteine ligase, the rate-limiting enzyme in glutathione biosynthesis.
• Also known as GCS light chain, gamma-ECS regulatory subunit, and gamma-glutamylcysteine synthetase regulatory subunit; encoded by the GCLM gene.
• Essential for cellular antioxidant defense; forms the holoenzyme with the catalytic subunit GCLC.
• Ubiquitously expressed across tissues, with highest levels observed in skeletal muscle.
• Subject to acetylation and alternative splicing, as identified by proteomics (UniProt keyword: Acetylation, Alternative splicing).
• Reference proteome protein with a predicted molecular weight of ~31 kDa (observed at 31 kDa).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the full-length human GCLM (aa 1-274), likely providing broad epitope coverage.
• For Western blot, the antibody detects a band at ~31 kDa in human, mouse, and rat lysates; optimal dilution should be determined experimentally.
• For immunofluorescence (IF/ICC), fixation and permeabilization conditions may require optimization; nuclear and cytoplasmic staining may be observed consistent with dual localization.
• For ELISA, the polyclonal nature may contribute to strong signal; consider cross-reactivity testing if using with rodent samples.
• Titrate the antibody and include appropriate positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and labs a streamlined sourcing solution for high-quality IVD raw materials, including this anti-GCLM polyclonal antibody for oxidative stress and glutathione metabolism research. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting seamless integration from concept to clinical application.