Anti-GCLM Rabbit Polyclonal Antibody for WB and ELISA - P48507

Core Product Specifications and Parameters

Biological Background: GCLM Function and Localization

• GCLM (glutamate-cysteine ligase modifier subunit) is the regulatory subunit of glutamate-cysteine ligase (GCL), the rate-limiting enzyme in glutathione (GSH) biosynthesis.
• It is also known as GLCLR, GCS light chain, or gamma-ECS regulatory subunit, reflecting its role in modulating GCL activity.
• GSH is a critical tripeptide antioxidant that protects cells from oxidative damage, regulates redox-sensitive signaling pathways, and participates in detoxification of xenobiotics.
• UniProt indicates ubiquitous expression of GCLM across human tissues, with particularly high levels in skeletal muscle, suggesting tissue-specific demand for glutathione.
• Post-translational modifications such as acetylation may influence protein stability, subcellular localization, or enzymatic function.
• Alternative splicing of the GCLM gene produces multiple transcript variants, which could generate functionally distinct protein isoforms.
• As a reference proteome component and a target in proteomics studies, GCLM is a key marker in investigations of oxidative stress and cellular defense.
• Keywords associated with GCLM include glutathione biosynthesis, acetylation, alternative splicing, and reference proteome, highlighting its central role in cellular redox homeostasis.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human GCLM (residues 1–274), so the antibody may recognize all splice variants containing this region. Validate specificity for your isoform of interest in biologically relevant samples.
• For Western blot, a single band at approximately 31 kDa is expected under reducing conditions. Include appropriate positive (e.g., skeletal muscle lysate) and negative controls, and optimize primary antibody dilution for your detection system.
• This antibody is reactive with human and mouse GCLM. Cross-reactivity with other species has not been tested; verify before use in non-human, non-mouse models.
• In ELISA applications, immobilize recombinant GCLM or cell/tissue extracts and detect with the antibody. Consider pairing with a GCL catalytic subunit (GCLC) antibody for sandwich assays if studying holoenzyme interactions.
• Store at -20°C upon receipt, aliquot to minimize freeze-thaw cycles, and avoid prolonged exposure to ambient temperatures as the product is shipped on ice bags.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-GCLM antibody, to advance your glutathione and redox biology research. From validated antibody pairs and bulk monoclonal/polyclonal reagents to ancillary buffers and rare-target sourcing, we support diagnostic manufacturers and laboratories in building reliable assays. Let CamelBio be your bridge from discovery to deployment, with technical consultation every step of the way.