Anti-GCLM Rabbit Monoclonal Antibody for WB, ELISA - P48507

Core Product Specifications and Parameters

Biological Background: GCLM Function and Localization

• The GCLM gene encodes the glutamate-cysteine ligase regulatory subunit, also known as the GCS light chain, Gamma-ECS regulatory subunit, or glutamate-cysteine ligase modifier subunit.
• Glutathione biosynthesis is a key biological process involving this protein, as indicated by its annotation with the corresponding keyword.
• The protein is widely expressed in human tissues, with the highest expression levels observed in skeletal muscle.
• Alternative splicing produces multiple transcript variants, potentially contributing to functional diversity.
• Post-translational modifications include acetylation, as listed in the protein keywords.
• The protein has a calculated molecular weight of approximately 31 kDa (30,727 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 175–274 of human GCLM; this internal region may balance accessibility and specificity.
• For Western blotting, expect a band near 31 kDa; confirm identity using positive controls (e.g., GCLM-overexpressing lysates) and negative controls (e.g., siRNA knockdown).
• In ELISA, consider immobilizing the peptide antigen to assess binding, and always include blank and control wells to minimize background.
• Since this is a monoclonal antibody, it recognizes a single epitope; if multiple bands appear, investigate whether they correspond to splice variants or degradation products.
• Optimal working dilutions and incubation conditions must be determined empirically for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering a comprehensive selection of validated antibodies and ancillary reagents. This GCLM monoclonal antibody supports research into glutathione metabolism and oxidative stress; CamelBio can supply complementary antibody pairs, bulk formats, and custom sourcing for rare targets. Our integrated services help diagnostic manufacturers and laboratories progress efficiently from concept to clinical application.