Anti-GCLC Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P48506

Core Product Specifications and Parameters

Biological Background: GCLC Function and Localization

• GCLC encodes the catalytic subunit of glutamate-cysteine ligase (GCL), the rate-limiting enzyme in glutathione biosynthesis.
• The enzyme catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine to form gamma-glutamylcysteine.
• GCLC is also referred to as GCL, GCS, GLCL, and GLCLC, reflecting its historical naming.
• Glutathione, the downstream product, is a critical intracellular antioxidant and detoxification agent.
• The protein contains ATP-binding and nucleotide-binding domains essential for its catalytic mechanism.
• Post-translational modifications such as acetylation and phosphorylation may modulate enzyme activity.
• Mutations in GCLC are linked to hereditary hemolytic anemia due to impaired glutathione synthesis.
• As a ligase in glutathione biosynthesis, GCLC is a key target for oxidative stress and drug resistance research.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is 73kDa; a 10% SDS-PAGE gel may be suitable.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization conditions for your cell type.
• As a polyclonal antibody, it can be used in ELISA; consider testing both capture and detection configurations.
• Validate the antibody in your specific sample system, as cross-reactivity has only been confirmed for human, mouse, and rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic manufacturers and researchers studying glutathione metabolism by offering this validated anti-GCLC polyclonal antibody along with complementary raw materials. Our portfolio includes optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—bridging your workflow from concept to clinic.