Anti-GCLC Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P48506

Core Product Specifications and Parameters

Biological Background: GCLC Function and Localization

• GCLC is the catalytic subunit of glutamate-cysteine ligase (GCL), the first and rate-limiting enzyme in glutathione (GSH) biosynthesis. It catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine to form gamma-glutamylcysteine, a critical antioxidant precursor.
• Alternative names include GCS heavy chain, Gamma-ECS, and Gamma-glutamylcysteine synthetase, reflecting its essential role in cellular redox homeostasis.
• The enzyme functions as a heterodimer with a regulatory subunit (GCLM), and its activity is tightly regulated to maintain intracellular GSH levels.
• Post-translational modifications such as acetylation and phosphorylation may modulate its activity, though specific roles require further investigation.
• Genetic variants in GCLC are associated with hereditary hemolytic anemia, underscoring its importance in protecting erythrocytes from oxidative stress.
• Identified as a proteomics reference target, GCLC is a key node in sulfur amino acid metabolism and detoxification pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 538-637 of human GCLC. This region lies near the C-terminus and was chosen for antigenicity; consider immunogen specificity when interpreting results.
• Validated for Western blotting (WB), immunohistochemistry of paraffin-embedded tissues (IHC-P), and ELISA. For WB, a band around 73 kDa is expected; however, validation in the relevant sample system is recommended.
• For species cross-reactivity, the antibody detects human and rat GCLC. Additional species may require sequence homology analysis.
• Optimize antibody dilution and experimental conditions according to your specific assay and sample type; reported applications are suggested starting points.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a broad portfolio of IVD raw materials, technical services, and consulting. The GCLC Rabbit mAb supports research into glutathione metabolism, oxidative stress, and related diseases. As your one-stop sourcing partner, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your projects from concept to clinic.