Anti-GCLC Monoclonal Antibody for WB, IF/ICC, ELISA - P48506

Core Product Specifications and Parameters

Biological Background: GCLC Function and Localization

• GCLC, also known as GCS heavy chain, gamma-ECS, or gamma-glutamylcysteine synthetase, is the catalytic subunit of glutamate--cysteine ligase (GCL).
• It catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine, the first and rate-limiting step in glutathione (GSH) biosynthesis, a critical antioxidant pathway.
• Together with the regulatory subunit GCLM, GCLC forms the heterodimeric GCL holoenzyme, which is essential for maintaining cellular redox homeostasis.
• Keywords indicate that GCLC is subject to acetylation and phosphorylation, suggesting regulation by post-translational modifications.
• Mutations in GCLC are associated with hereditary hemolytic anemia due to glutathione deficiency, underscoring its clinical relevance.
• GCLC contains an ATP-binding domain, consistent with its role as a ligase that uses ATP for glutathione synthesis.
• It is a member of the reference proteome and has been identified in multiple proteomics studies, confirming its ubiquitous expression.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-135) of human GCLC, a region that may be conserved across species, consistent with the observed cross-reactivity with mouse.
• For western blot (WB), consider optimizing primary antibody dilution and validate with positive and negative control lysates expressing GCLC.
• In immunofluorescence / immunocytochemistry (IF/ICC), ensure proper fixation and permeabilization methods suitable for your cell type; inclusion of isotype controls is recommended.
• For ELISA, test against recombinant GCLC or native protein in cell/tissue lysates; a checkerboard titration may help determine optimal antibody and antigen concentrations.
• Given the cross-reactivity with mouse, this antibody may be suitable for translational studies in rodent models; validation in the relevant species is advised.
• Always include appropriate controls (e.g., knockout samples or siRNA knockdown) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, offering validated antibody pairs and high-quality monoclonal antibodies like this anti-GCLC clone for glutathione metabolism and oxidative stress research. We supply bulk ancillary reagents and specialize in sourcing rare-target raw materials, streamlining your transition from concept to clinic. Partner with CamelBio for efficient, end-to-end support in diagnostic development.