Anti-GCKR Polyclonal Antibody for WB, ELISA - Q14397

Core Product Specifications and Parameters

Biological Background: GCKR Function and Localization

• GCKR binds and inhibits glucokinase (GCK) by forming an inactive complex, thereby regulating glucose phosphorylation. Related references: PMID:23621087 PMID:23733961
• It promotes GCK recruitment to the nucleus, providing a reserve that can be quickly released into the cytoplasm after a meal. Related references: PMID:10456334
• The GCKR-GCK interaction is allosterically modulated by fructose metabolites: fructose 6-phosphate increases GCK binding and inhibition, whereas fructose 1-phosphate decreases affinity and relieves inhibition. Related references: PMID:23621087 PMID:23733961
• GCKR is primarily expressed in liver and pancreas, with no detectable levels in other tested tissues.
• Subcellularly, GCKR is found in the cytoplasm, nucleus, and mitochondria.
• The protein contains multiple repeat regions and undergoes alternative splicing, and its crystallographic structure has been determined; it is annotated with keywords including 3D-structure, Alternative splicing, Carbohydrate metabolism, and Repeat.

Experimental Guidance and Technical Tips

• Since the antibody is raised against a recombinant protein covering the N-terminal 1-300 amino acids of human GCKR, it may detect full-length GCKR and potentially truncated isoforms. For WB, a band around 69 kDa is expected; consider using appropriate controls and validate in hepatic or pancreatic cell lines or tissue lysates where GCKR is expressed.
• For ELISA, ensure the antibody pair is validated for sandwich immunoassay if used as capture or detection; the polyclonal nature may provide high sensitivity but consider cross-reactivity with related proteins.
• Given cross-reactivity with mouse and rat, this antibody can be used for studies in rodent models. Validate in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research on glucose metabolism and glucokinase regulation, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us for procurement support tailored to your IVD development needs.