Anti-GCC2 / GCC185 Rabbit Polyclonal Antibody for WB, IHC-P and ELISA - Q8IWJ2

Core Product Specifications and Parameters

Biological Background: GCC2 Function and Localization

• GCC2 encodes GRIP and coiled-coil domain-containing protein 2, also known as GCC185, RANBP2L4, and renal carcinoma antigen NY-REN-53.
• It functions as a Golgin that tethers transport vesicles to the trans-Golgi network (TGN) and acts as a RAB9A effector to regulate the recycling of the mannose 6-phosphate receptor from late endosomes to the TGN.
• GCC2 is required for the maintenance of Golgi structure and the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2.
• The protein localizes to the cytoplasm, Golgi apparatus, and specifically the trans-Golgi network membrane.
• Ubiquitously expressed across human tissues.
• Post-translational modifications include phosphorylation and acetylation; multiple protein isoforms are generated via alternative splicing.
• With a predicted molecular weight of ~196 kDa (195,910 Da), GCC2 is a large coiled-coil membrane trafficking protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-300) of human GCC2; the resulting polyclonal antibody may recognize multiple epitopes.
• For Western blot, expect a band at approximately 196 kDa under reducing conditions; validate with appropriate positive and negative controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval methods and antibody dilution using known positive tissue sections (e.g., human or mouse).
• For ELISA applications, the recombinant immunogen can be used as a positive control to assess antibody reactivity.
• Due to its polyclonal nature, lot-to-lot consistency should be verified for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. For Golgi biology and membrane trafficking studies, we supply GCC2 antibodies, validated antibody pairs, and bulk ancillary reagents to streamline your assay development. Contact us for sourcing rare targets and tailored procurement solutions.