Anti-GCAT Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75600

Core Product Specifications and Parameters

Biological Background: GCAT Function and Localization

• GCAT encodes the mitochondrial enzyme 2-amino-3-ketobutyrate coenzyme A ligase, also known as aminoacetone synthase or glycine acetyltransferase.
• This pyridoxal phosphate (PLP)-dependent transferase catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA, a key step in threonine degradation and glycine biosynthesis.
• GCAT localizes predominantly to the mitochondrion but has also been detected in the nucleus.
• The protein is strongly expressed in heart, brain, liver, and pancreas, with additional expression in lung.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.
• UniProt keywords highlight roles in acyltransferase activity, PLP-dependent catalysis, and mitochondrial protein import via a transit peptide.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 170-419 of human GCAT, covering a large portion of the protein; therefore, the antibody may recognize multiple isoforms.
• For Western blotting, a band corresponding to the predicted molecular weight of approximately 45 kDa is expected; verify absence in knockout controls if available.
• In IHC-P, positive staining is anticipated in tissues with high expression such as heart, liver, brain, and pancreas; include omission of primary antibody as negative control.
• When using for IF/ICC, mitochondrial localization suggests mild permeabilization may suffice, but dual labeling with mitochondrial markers is recommended to confirm specificity.
• For ELISA applications, the recombinant immunogen or purified target protein can serve as coating antigen; empirical optimization of coating concentration and blocking conditions is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials and technical services, supporting diagnostic developers from concept to clinic. This anti-GCAT antibody is suitable for research into mitochondrial metabolism and glycine biosynthesis pathways. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents, along with sourcing capabilities for rare target raw materials to streamline your assay development.