Anti-GC1q R/C1QBP Polyclonal Antibody for WB, IF/ICC, ELISA - Q07021

Core Product Specifications and Parameters

Biological Background: GC1q R/C1QBP Function and Localization

• GC1q R/C1QBP is a multifunctional and multicompartmental protein involved in inflammation, ribosome biogenesis, mitochondrial protein synthesis, apoptosis, transcriptional regulation, and pre-mRNA splicing. Related references: PMID:10022843, PMID:10479529, PMID:10722602

• At the cell surface, it serves as a receptor for complement C1q, binding its globular heads and inhibiting C1 activity, potentially in a complex with C1qR/CD93. Related references: PMID:8195709, PMID:20810993

• It also acts as a high-affinity receptor for kininogen-1/HMWK in complex with cytokeratin-1/KRT1 and can bind coagulation factor XII, linking it to the kallikrein-kinin and coagulation pathways. Related references: PMID:21544310

• Within mitochondria, it is essential for protein synthesis and formation of functional 55S mitoribosomes, functioning as an RNA modification reader that recognizes m5C-modified mitochondrial RNAs and promotes their degradation. Related references: PMID:28942965, PMID:39019044

• It regulates pre-mRNA splicing by inhibiting the RNA-binding capacity and phosphorylation of SRSF1, and is required for nuclear translocation of U2AF1L4. Related references: PMID:10022843, PMID:21536856

• The protein also modulates apoptosis by stabilizing the mitochondrial CDKN2A isoform smARF and plays a role in antibacterial defense by binding hyaluronan and inhibiting Streptococcus pneumoniae hyaluronate lyase. Related references: PMID:17486078, PMID:19004836

• GC1q R/C1QBP localizes to multiple compartments including the mitochondrial matrix, nucleus, nucleolus, cell membrane, cytoplasm, and is secreted.

• It is expressed on the surface of macrophages and monocyte-derived dendritic cells, and is involved in viral replication and host-pathogen interactions, serving as an attachment site for various microbial proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 74–282 of human GC1q R/C1QBP, covering the mature protein sequence. This region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, the predicted molecular weight is 31 kDa, but the mature mitochondrial form may appear around 33 kDa. Optimize blocking and antibody dilution for your specific sample.
• Suitable for immunofluorescence/ICC; consider permeabilization protocols appropriate for the target subcellular localization (mitochondrial, nuclear, cytoplasmic, or membrane).
• For ELISA applications, validate the antibody pair with the relevant recombinant protein or biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-GC1q R/C1QBP antibody is part of our complement and mitochondrial biology collection, with bulk supply and custom sourcing available for rare targets. Contact us to discuss your project-specific needs.