Anti-GBX2 Polyclonal Antibody for WB, ELISA - P52951

Core Product Specifications and Parameters

Biological Background: GBX2 Function and Localization

• GBX2 (Homeobox protein GBX-2) is a member of the homeobox family of DNA-binding transcription factors.
• Also known as Gastrulation and brain-specific homeobox protein 2, it may regulate genes involved in cell pluripotency and embryonic differentiation.
• GBX2 plays a critical role in gastrulation and brain development, acting as a transcription factor in early embryogenesis.
• The protein contains a conserved homeodomain that mediates sequence-specific DNA binding, a hallmark of this gene family.
• Localized predominantly in the nucleus, consistent with its function in transcriptional regulation.
• Post-translational modifications and interaction partners may further modulate its activity, as indicated by proteomics identification.
• GBX2 is recognized as a reference proteome component, underscoring its fundamental biological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the near full-length human GBX2 protein, suggesting the polyclonal antibody may recognize multiple epitopes. Consider using nuclear-enriched lysates for improved detection in WB, given its nuclear localization.
• For WB, test the antibody with positive controls from human, mouse, or rat cell lines known to express GBX2, and validate bands at the expected 37 kDa. Specificity can be confirmed using siRNA knockdown or knockout models.
• In ELISA, the antibody can be employed to quantify GBX2 in biological samples, but assay conditions (coating antigen, blocking, and detection system) should be optimized empirically.
• As with any polyclonal reagent, lot-to-lot variability may occur; it is advisable to test each new batch in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. For transcription factor and developmental biology projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our services also include rare target raw-material sourcing to streamline your assay development.