Anti-GBF1 Rabbit Polyclonal Antibody for WB, ELISA - Q92538

Core Product Specifications and Parameters

Biological Background: GBF1 Function and Localization

• GBF1 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1) is a 206 kDa protein encoded by the GBF1 gene (Gene ID: 8729). It belongs to the family of guanine-nucleotide exchange factors (GEFs) for Arf small GTPases.
• Functions as a GEF for ARF1, ARF4, and ARF5, catalyzing GDP to GTP exchange to activate Arf proteins, which initiates COPI vesicle coating at early secretory compartments. Related references: PMID:12047556, PMID:12808027, PMID:15616190
• Recruited to cis-Golgi membranes in an Arf-GDP-dependent manner and is essential for COPI coat recruitment at ER exit sites (ERES), ERGIC, and cis-Golgi compartments, mediating retrograde transport to the ER. Related references: PMID:12047556, PMID:12808027, PMID:16926190
• Involved in trans-Golgi network recruitment of clathrin adaptors (GGA1-3, BIG1/2, AP-1) through GEF activity on ARF4/ARF5, facilitating clathrin-dependent transport. Related references: PMID:23386609
• Required for Golgi apparatus assembly and maintenance; phosphorylation by AMPK triggers Golgi disassembly during mitosis and stress. Related references: PMID:12808027, PMID:18003980, PMID:18063581, PMID:23418352
• Subcellularly localized to the Golgi apparatus (cis- and trans-Golgi), ERGIC, cytoplasm, lipid droplets, and membrane.
• Ubiquitously expressed and linked to Charcot-Marie-Tooth disease type 2GG (CMT2GG) when mutated.
• May participate in G protein-coupled receptor (GPCR)-mediated chemotaxis and superoxide production in neutrophils by recruiting ARF1 and NADPH oxidase complex to the leading edge. Related references: PMID:22573891
• Plays a role in maintaining mitochondrial morphology. Related references: PMID:25190516

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-85) of human GBF1, suggesting the antibody may recognize epitopes within this domain.
• For Western blot, use a high-molecular-weight gel (e.g., 8% SDS-PAGE) and optimize transfer conditions for the large 206 kDa protein.
• Validate antibody specificity in relevant human and mouse cell/tissue lysates; consider using siRNA knockdown or overexpression controls.
• For ELISA, the antibody can serve as a capture or detection reagent; titrate to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for sourcing high-quality IVD raw materials, from validated antibodies to bulk ancillary reagents. This anti-GBF1 polyclonal antibody supports research on Golgi trafficking and related diseases, complementing our extensive portfolio of antibodies for cell biology and neurological disorder targets. Whether you need optimized antibody pairs or rare-target raw materials, CamelBio bridges your procurement needs from concept to clinic.