Anti-GBE1 Rabbit Polyclonal Antibody for WB, ELISA - Q04446

Core Product Specifications and Parameters

Biological Background: GBE1 Function and Localization

• Encodes 1,4-alpha-glucan-branching enzyme (glycogen-branching enzyme), also known by aliases GBE, APBD, and GSD4.
• Functions in glycogen biosynthesis together with glycogenin and glycogen synthase.
• Catalyzes the transfer of alpha-1,4-linked glucose chains to form alpha-1,6-glucosidic branches, increasing glycogen solubility. Related references: PMID:26199317, PMID:8463281, PMID:8613547
• Mutations are linked to glycogen storage disease type IV (Andersen disease); keywords include disease variant and glycogen storage disease.
• Post-translational modifications include acetylation and phosphorylation (keywords: Acetylation, Phosphoprotein).
• Predicted molecular weight is ~80 kDa (UniProt: 80474 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-300 (NP_000149.3), an N-terminal region that may recognize multiple isoforms or processed forms; validation in the target sample system is recommended.
• For Western blot, anticipate a main band near 80 kDa; additional bands may arise from modifications or degradation — optimize sample preparation and loading.
• For ELISA, the polyclonal antibody can be used as capture or detection reagent; pairing with a matched monoclonal or polyclonal antibody may improve sensitivity but requires empirical testing.
• As a polyclonal reagent, lot-to-lot consistency should be confirmed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive IVD raw-material procurement platform, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For glycogen metabolism targets like GBE1, we supply this rabbit polyclonal antibody and can assist with sourcing rare-target reagents for assay development. Our integrated technical services and consulting support your journey from concept to clinic.