Anti-GBA2 Rabbit Polyclonal Antibody for WB and ELISA - Q9HCG7

Core Product Specifications and Parameters

Biological Background: GBA2 Function and Localization

• GBA2 (non-lysosomal glucosylceramidase, also known as beta-glucocerebrosidase 2) catalyzes the hydrolysis of glucosylceramides into glucose and ceramide. Related references: PMID:17105727, PMID:30308956, PMID:32144204
• It exhibits transglucosylation activity, transferring glucose from glucosylceramide to cholesterol, thereby modifying cholesterol's solubility and biological properties. Related references: PMID:32144204
• The enzyme also hydrolyzes galactosylceramides and can mediate galactosyl transfer between galactosylceramides and cholesterol, albeit with lower activity. Related references: PMID:32144204
• GBA2 plays a role in bile acid metabolism, capable of hydrolyzing bile acid 3-O-glucosides and producing bile acid-glucose conjugates. Related references: PMID:11489889, PMID:17080196, PMID:9111029
• The protein localizes to the endoplasmic reticulum and Golgi apparatus membranes.
• Widely expressed, with highest levels in brain, heart, skeletal muscle, kidney, and placenta; lower in liver, spleen, small intestine, and lung; also detectable in colon, thymus, and peripheral blood leukocytes. Related references: PMID:11489889
• Keywords include alternative splicing, disease variant (SPG46), hereditary spastic paraplegia, and neurodegeneration; GBA2 mutations are linked to lipid metabolism disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 708-927); the antibody likely recognizes an epitope in this region. For Western blot, consider using reducing and non-reducing conditions to assess potential conformational epitopes.
• Suitable for ELISA applications; optimize coating conditions and consider using recombinant GBA2 protein or cell lysates from GBA2-expressing tissues as the antigen.
• Cross-reactivity with mouse enables validation in mouse models. Validate in relevant sample systems such as brain, heart, or muscle tissues where GBA2 expression is high.
• The target protein has a predicted molecular weight of approximately 105 kDa; verify band size and consider possible glycosylation or degradation products that may shift migration.

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