Anti-GATM Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50440

Core Product Specifications and Parameters

Biological Background: Glycine Amidinotransferase Function and Localization

• GATM (glycine amidinotransferase, also known as AGAT or transamidinase) is a mitochondrial enzyme that catalyzes the transfer of an amidino group from L-arginine to glycine, the rate-limiting step of creatine biosynthesis. Related references: PMID:27233232, PMID:36543883, PMID:3800397
• In addition to glycine, GATM can use beta-alanine, GABA, and taurine as amidino acceptors, producing various guanidine derivatives. Related references: PMID:16820567
• It localizes predominantly to the mitochondrial inner membrane, with some cytoplasmic presence.
• Tissue expression is highest in kidney, but it is also found in brain, heart, liver, lung, salivary gland, and skeletal muscle; biallelic expression occurs in placenta and fetal tissues.
• Alternative splicing generates multiple isoforms, and post-translational modifications include acetylation and phosphorylation; mutations in GATM are associated with human diseases.

Experimental Guidance and Technical Tips

• The immunogen spans a large region (aa 38-423), so the antibody may recognize multiple isoforms and species homologs; validate specificity in your target tissue or cell line.
• For Western blotting, the predicted molecular weight of human GATM is ~48 kDa; include appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; optimize using known positive tissues such as kidney.
• For immunofluorescence (IF/ICC), mitochondrial localization requires permeabilization; consider using MitoTracker co-staining to confirm.
• For ELISA, the recombinant protein used as immunogen can serve as a standard if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This GATM antibody is part of our extensive portfolio for metabolic and mitochondrial research, and we also provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For rare or custom targets, our sourcing team ensures reliable, scalable supply to accelerate your assay development.