Anti-GATC Polyclonal Antibody for WB, ELISA - O43716

Core Product Specifications and Parameters

Biological Background: GATC Function and Localization

• GATC encodes the mitochondrial glutamyl-tRNA(Gln) amidotransferase subunit C, also known as Protein 15E1.2.
• It mediates the transamidation of misacylated Glu-tRNA(Gln) to form correctly charged Gln-tRNA(Gln) in mitochondria, an ATP-dependent process.
• This enzyme is essential for mitochondrial protein biosynthesis.
• The protein localizes to the mitochondrion.
• GATC is associated with primary mitochondrial disease, and pathogenic variants have been identified.
• It contains ATP-binding and nucleotide-binding domains, characteristic of ligases.
• The molecular weight of the mature protein is approximately 15 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-136 of human GATC, which may target multiple isoforms or conformational epitopes. Consider this when designing experiments.
• For Western blotting (WB), the predicted molecular weight is 15 kDa; ensure adequate separation in SDS-PAGE and optimize transfer conditions.
• For ELISA, validate antibody specificity in the relevant sample matrix and consider cross-reactivity with mouse and rat orthologs.
• Since GATC is a mitochondrial protein, sample preparation may require mitochondrial enrichment for optimal detection.
• Use standard positive and negative controls to confirm antibody performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, to support your diagnostic and research programs. This anti-GATC polyclonal antibody is well-suited for studying mitochondrial protein synthesis and tRNA modification, essential for understanding primary mitochondrial diseases. As your one-stop sourcing partner, CamelBio also offers rare target raw-material sourcing and technical services to streamline your assay development from concept to clinic.