Anti-GATAD2B Rabbit Polyclonal Antibody (p66-beta) for WB, IHC-P, IP, ELISA - Q8WXI9

Core Product Specifications and Parameters

Biological Background: GATAD2B Function and Localization

• GATAD2B (Transcriptional repressor p66-beta, also known as p66/p68) is a GATA zinc finger domain-containing protein that functions as a transcriptional repressor.
• It acts as a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which participates in chromatin remodeling and transcriptional silencing. Related references: PMID:16428440 | PMID:28977666
• GATAD2B enhances MBD2-mediated transcriptional repression, and efficient repression requires the presence of GATAD2A. Related references: PMID:12183469 | PMID:16415179
• It targets MBD3 to discrete loci in the nucleus. Related references: PMID:11756549
• The protein localizes to nuclear speckles, the nucleus, and chromosomes.
• GATAD2B may play a role in synapse development. Related references: PMID:23644463
• It is subject to phosphorylation and ubiquitination, and contains zinc-finger motifs; mutations are associated with intellectual disability.
• Widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 120 amino acids of human GATAD2B; this region may be useful for detecting total GATAD2B protein across species where sequence homology is high.
• For western blotting, a band around 65 kDa is expected; consider using appropriate loading controls and validating in your specific cell or tissue lysates.
• For immunohistochemistry (paraffin), optimize antigen retrieval and antibody dilution; note that GATAD2B localizes to the nucleus, so nuclear staining is anticipated.
• For immunoprecipitation and ELISA, standard protocols may be adapted; cross-reactivity with human, mouse, and rat is reported, but independent validation in the target species and application is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, providing diagnostic manufacturers and researchers with validated antibodies, recombinant proteins, and rare-target sourcing. This anti-GATAD2B antibody serves as a tool for studying chromatin remodeling and transcriptional regulation, and our team can support your assay development needs from custom immunogens to bulk supply.