Anti-GATA6 Polyclonal Antibody for WB, ELISA - Q92908

Core Product Specifications and Parameters

Biological Background: GATA6 Function and Localization

• Transcription factor GATA-6 (GATA-binding factor 6) functions as a transcriptional activator, regulating genes such as SEMA3C and PLXNA2, and is involved in BMP-mediated cardiac-specific gene expression. Related references: PMID:19666519, PMID:22750565, PMID:22824924

• GATA6 regulates gene expression in the gastric epithelium and may protect epithelial cells from bacterial infection. Related references: PMID:9315713, PMID:16968778

• In human skin, GATA6 controls sebocyte differentiation, proliferation, and lipid production, mediates retinoic acid effects, and modulates immune and antimicrobial responses. Related references: PMID:33082341

• GATA6 binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions, contributing to heart development (By similarity).

• Predominantly localized in the nucleus, consistent with its role as a DNA-binding transcription factor.

• Contains two C2H2-type zinc-finger domains, facilitating sequence-specific DNA binding and transcriptional regulation; subject to post-translational modifications such as phosphorylation and ubiquitin conjugation.

• Expressed in heart, gut, and gut-derived tissues; expression is decreased or lost in acne lesions (as reported in skin pilosebaceous units). Related references: PMID:33082341

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 70-160 of human GATA6, a region N-terminal to the first zinc finger. This sequence is relatively conserved between human and mouse, consistent with the observed cross-reactivity. When using this antibody, verify specificity in the relevant species and sample type.
• For Western blot, a band at approximately 60 kDa is predicted; however, post-translational modifications or alternative initiation may result in shifts. Optimize loading and transfer conditions for nuclear proteins.
• In ELISA applications, consider employing the recombinant immunogen as a positive control to assess antibody binding and titer.
• Given GATA6's nuclear localization, use appropriate lysis buffers and sample preparation protocols to ensure efficient extraction of the target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated polyclonal antibodies such as this anti-GATA6 pAb for transcription factor research. Beyond individual reagents, we supply optimized antibody pairs, monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets in gene regulation and epithelial biology. Empower your assay development and diagnostic projects with CamelBio’s comprehensive raw-material solutions, covering every stage from concept to clinic.