Anti-GATA4 Polyclonal Antibody for WB, IHC, IF/ICC and ELISA - P43694

Core Product Specifications and Parameters

Biological Background: GATA4 Function and Localization

• GATA4 (GATA-binding factor 4) is a zinc-finger transcription factor that binds the consensus sequence 5'-AGATAG-3' and is essential for cardiac development and function. Related references: PMID:24000169, PMID:27984724, PMID:35182466

• In cooperation with TBX5, GATA4 regulates cardiac super-enhancers, driving cardiomyocyte gene expression and suppressing endocardial genes. Related references: PMID:27984724

• GATA4 mediates bone morphogenetic protein (BMP)-induced cardiac gene expression by binding to BMP response elements within cardiac activating regions.

• Acts as a transcriptional activator of ANF (atrial natriuretic factor) in synergy with NKX2-5.

• GATA4 promotes cardiac myocyte enlargement, contributing to hypertrophic responses. Related references: PMID:20081228

• GATA4 is essential for testicular development. Related references: PMID:21220346

• May participate in sphingolipid signaling by controlling the expression of sphingosine-1-phosphate lyase. Related references: PMID:15734735

• GATA4 is a nuclear protein with two C-terminal zinc-finger domains; it undergoes alternative splicing and post-translational modifications, including methylation.

Experimental Guidance and Technical Tips

• For Western blot, an expected band of approximately 45 kDa may be observed; use a cardiac tissue lysate as a positive control to confirm detection.
• In immunofluorescence and immunohistochemistry (including paraffin sections), nuclear staining consistent with GATA4 localization should be seen; consider using a nuclear counterstain and heat-induced antigen retrieval for IHC-P.
• As a peptide-based polyclonal antibody, cross-adsorption or peptide blocking can be used to validate specificity in ELISA or IF assays.
• The immunogen sequence (aa 341-440, near the C-terminus) is conserved in human, mouse, and rat, supporting the observed cross-reactivity; nonetheless, verify reactivity in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research teams streamlined access to high-quality raw materials for immunoassay development. For studies of cardiac transcription factors like GATA4, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing services. From initial screening to clinical validation, our one-stop platform bridges the gap between discovery and diagnostic application.