Anti-GATA3 Rabbit PolymAb® for WB, IF/ICC, IF-P, IHC-P, ChIP, ELISA - P23772

Core Product Specifications and Parameters

Biological Background: GATA3 Function and Localization

• GATA3 (GATA-binding factor 3) is a transcriptional activator that binds to the enhancer of the T-cell receptor alpha and delta genes, recognizing the consensus sequence 5'-AGATAG-3'; it is required for T-helper 2 (Th2) differentiation during immune and inflammatory responses.
• Positively regulates ASB2 expression, linking to protein degradation pathways. Related references: PMID:31175139
• Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33, driving differentiation of inflammation-resolving alternatively activated macrophages upon tissue injury.
• Localizes to the nucleus, consistent with its DNA-binding transcription factor function.
• Exhibits T-cell-specific expression, critical for T-cell development and effector functions.
• Contains two C4-type zinc-finger domains that mediate DNA binding and transcriptional regulation.
• Post-translational modifications include phosphorylation, modulating its regulatory activity.
• Classified as a DNA-binding activator involved in immunity, innate immunity, and transcription regulation; a member of the GATA family of zinc-finger proteins.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 135-258 of mouse GATA3, including the zinc-finger DNA-binding domain; this region is highly conserved, and the antibody is likely to recognize the native protein in multiple applications.
• For Western blotting, expect a band near 48 kDa; consider using nuclear extracts as a positive control and validate with appropriate T-cell lysates.
• In chromatin immunoprecipitation (ChIP), verify binding efficiency by quantitative PCR at known GATA3 target loci, and optimize cross-linking conditions for your cell type.
• For immunofluorescence and immunohistochemistry, anticipate nuclear staining; always include a no-primary control and confirm specificity using Gata3-knockout models or siRNA-treated samples.
• Titrate the antibody for each application and validate in the relevant biological system (human, mouse, or rat) to establish optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. The anti-GATA3 rabbit PolymAb® supports T-cell research and Th2 differentiation studies, and CamelBio can source rare target raw materials or custom formulations to meet your specific immunoassay development needs. Partner with CamelBio to streamline your IVD project from concept to clinic.