Anti-GATA3 Rabbit Monoclonal Antibody for WB, IP, ELISA, ChIP, ChIP-seq - P23771

Core Product Specifications and Parameters

Biological Background: GATA3 Function and Localization

• GATA3 is a zinc-finger transcription factor that acts as a transcriptional activator, binding to the consensus DNA sequence 5'-AGATAG-3' to regulate the expression of T-cell receptor alpha and delta genes.
• It is essential for the T-helper 2 (Th2) cell differentiation process during immune and inflammatory responses.
• GATA3 positively regulates ASB2 expression, although the exact mechanism remains to be fully characterized (by similarity).
• In response to IL33 signaling, GATA3 coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming, promoting the differentiation of inflammation-resolving alternatively activated macrophages upon tissue injury.
• The protein is localized to the nucleus, consistent with its role as a transcription factor.
• GATA3 is predominantly expressed in T-cells and endothelial cells, highlighting its specialized roles in immune and vascular biology.
• Post-translational modifications, including phosphorylation, regulate its activity, and mutations in GATA3 are associated with human diseases such as deafness (HDR syndrome).
• Alternative splicing generates multiple isoforms, which may have distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human GATA3; consider that the antibody may detect multiple isoforms if the N-terminus is shared.
• For ChIP and ChIP-seq applications, optimize crosslinking and shearing conditions for your specific cell type to ensure robust enrichment of GATA3-bound genomic regions.
• Validate the antibody in your relevant sample system, such as T-cell lines or primary cells, as expression levels and modifications can vary.
• The detected band on WB is approximately 48 kDa; slight variations may occur due to post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide comprehensive IVD raw-material sourcing. For transcription-factor targets like GATA3, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to support your development from concept to clinic.