Anti-GATA2 Rabbit Polyclonal Antibody for IHC-P, ELISA - P23769

Core Product Specifications and Parameters

Biological Background: GATA2 Function and Localization

• GATA2, designated as endothelial transcription factor GATA-2, is a member of the GATA family of zinc-finger transcription factors (UniProt P23769).
• It acts as a transcriptional activator, known to regulate the expression of the endothelin-1 gene in endothelial cells.
• The protein binds to the consensus DNA sequence 5’-AGATAG-3’ via its two conserved zinc-finger domains.
• GATA2 localizes predominantly to the nucleus, consistent with its function in DNA binding and transcription regulation.
• Its expression is primarily observed in endothelial cells, underscoring its tissue-specific regulatory role.
• Post-translational modifications such as phosphorylation, methylation, and ubiquitination have been reported, potentially modulating its activity.
• Alternative splicing generates multiple isoforms, and disease-associated variants have been noted, highlighting clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from amino acids 240-340 of human GATA2 (NP_116027.2). This region may be conserved; verify reactivity in your target species.
• This antibody has been tested in IHC-P and ELISA; for other applications, optimization of dilution and conditions is advisable.
• For IHC-P, antigen retrieval methods may affect staining; standard citrate or EDTA-based protocols are often suitable.
• Include appropriate positive and negative controls, such as endothelial cell lines or tissue known to express GATA2.
• Ensure proper storage as shipped on ice bags; aliquot and store at -20°C or below to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-GATA2 product. We offer one-stop support from concept to clinic, with bulk ancillary reagents and rare target sourcing capabilities to streamline your development pipeline. Our expertise in molecular biology tools ensures reliable supply for your transcriptional research and endothelial biology studies.