Anti-GATA2 Polyclonal Antibody for WB, IHC-P, ELISA - P23769

Core Product Specifications and Parameters

Biological Background: GATA2 Function and Localization

• GATA2 is a zinc-finger transcription factor that binds specifically to the DNA consensus sequence 5'-AGATAG-3' (UniProt: P23769).
• It functions as a transcriptional activator, critically regulating endothelin-1 gene expression in endothelial cells.
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional control.
• GATA2 is primarily expressed in endothelial cells, where it plays essential roles in vascular development and hematopoiesis.
• Post-translational modifications, including phosphorylation, methylation, and ubiquitin-like conjugation, modulate its transcriptional activity and stability.
• Mutations in the GATA2 gene are associated with primary immunodeficiency syndromes such as DCML deficiency, MonoMAC syndrome, and IMD21, underscoring its importance in immune cell development.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-300 at the N-terminus; therefore, the antibody may recognize both full-length GATA2 and potential N-terminal isoforms or cleavage products. Verification in the relevant sample system is recommended.
• For Western blot, a band of approximately 51 kDa is expected. Consider using nuclear extracts from endothelial cell lines or GATA2-expressing tissues as positive controls.
• For immunohistochemistry (paraffin-embedded sections), antigen retrieval steps may be required. Tissues with high endothelial content (e.g., vascular structures) can serve as positive controls.
• In ELISA applications, pairing with another GATA2-specific antibody could be explored for sandwich assay development; validation of detection specificity and sensitivity is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering one-stop access to IVD raw materials, technical services, and consulting. This anti-GATA2 polyclonal antibody supports research into transcriptional regulation, vascular biology, and immunodeficiency disorders. Beyond individual antibodies, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material procurement to accelerate your assay development and production needs.