Anti-GATA1 Monoclonal Antibody for WB, IP, ELISA - P15976

Core Product Specifications and Parameters

Biological Background: GATA1 Function and Localization

• GATA1 (erythroid transcription factor, also known as GF-1, NF-E1, Eryf1) is a key regulator of erythroid development, belonging to the GATA family of zinc-finger transcription factors.
• It acts as a transcriptional activator or repressor, binding to consensus DNA sequences 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and other erythroid-specific genes. In K562 erythroleukemia cells, it activates transcription of HBB, HBG1/2, ALAS2 and HMBS. Related references: PMID:35030251 PMID:24245781
• The protein is localized exclusively to the nucleus.
• Expression is predominantly observed in erythrocytes.
• GATA1 is subject to acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, modulating its activity and stability.
• Mutations in GATA1 are associated with hereditary hemolytic anemia and other blood disorders.
• Structurally, GATA1 features two C4-type zinc-finger domains that mediate DNA binding and protein interactions.
• The gene produces multiple isoforms via alternative initiation and splicing, potentially yielding functionally distinct variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human GATA1 (NP_002040.1), targeting the N-terminal region. This antibody may recognize full-length GATA1 and isoforms retaining the N-terminus; verify specificity in your system.
• For western blotting, a band of approximately 43 kDa is expected; include suitable positive and negative controls, and normalize against a loading control.
• In immunoprecipitation, optimize antibody concentration, incubation time, and lysis buffer conditions to ensure efficient capture of nuclear GATA1.
• As a rabbit monoclonal antibody, it typically offers high specificity and lot-to-lot consistency; validate in your specific application and sample type before scaling up.
• This antibody has been reported to cross-react with human GATA1; reactivity with other species should be independently confirmed if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-GATA1 antibody, ideal for erythropoiesis research and hematological disorder assays. Beyond single products, we offer matched antibody pairs, bulk ancillary reagents, and sourcing solutions for rare targets, backed by comprehensive technical and consulting support from concept to clinic. Partner with CamelBio to streamline your IVD development with reliable, scalable raw material supply.