Anti-Gastrin Releasing Peptide (GRP) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07492

Core Product Specifications and Parameters

Biological Background: Gastrin-releasing peptide Function and Localization

• Encodes the gastrin-releasing peptide, a secreted neuropeptide that stimulates the release of gastrin and other gastrointestinal hormones (By similarity).
• Acts as a key mediator of nonhistaminergic itch sensation; contributes to the perception of pruritic stimuli and transmission of itch signals in the spinal cord, and can induce mast cell degranulation.
• Plays a role in fear memory processing by modulating neuronal activity in the amygdala and auditory cortex; enhances auditory fear memories via GRPR signaling on VIP-expressing cells (By similarity).
• Also implicated in feeding behavior, respiratory control (sighing), and modulation of neuronal ion channels (By similarity).
• Undergoes amidation and proteolytic cleavage on pairs of basic residues to generate bioactive peptide forms.
• Localized to secretory vesicles and secreted; found in neuron projections.
• The GRP gene undergoes alternative splicing.
• Predicted molecular weight is approximately 16.2 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human GRP precursor (aa 1-148); therefore, the antibody may recognize both the precursor and mature peptide forms. Consider appropriate controls for post-translational processing.
• For Western blot, expect a band near 16 kDa (predicted size of the precursor). Additional lower molecular weight bands may represent processed forms.
• For immunohistochemistry and immunofluorescence, validate the antibody in relevant tissue types known to express GRP, such as gastrointestinal tract, spinal cord, and brain regions (amygdala, auditory cortex).
• For ELISA, use purified recombinant GRP or synthetic peptides as standards; note that the polyclonal nature may necessitate careful optimization of detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD-grade antibodies, antigens, and ancillary reagents. With a focus on gastrointestinal hormones, neuropeptides, and itch/pain research, we offer validated antibody pairs, monoclonal/polyclonal antibodies, and custom sourcing for rare targets. Streamline your assay development with CamelBio’s integrated supply chain and technical support.