Anti-Gasdermin B (Full Length + C terminal) Rabbit Polyclonal Antibody for Western Blot - Q8TAX9-6

Core Product Specifications and Parameters

Biological Background: Gasdermin B Function and Localization

• Gasdermin-B (GSDMB), also known as Gasdermin-like protein (GSDML), is encoded by the GSDMB gene and belongs to the gasdermin family of pore-forming proteins.
• Isoform 6 of GSDMB (UniProt: Q8TAX9-6) is a precursor that, upon proteolytic activation, forms pores in the plasma membrane to execute pyroptosis.
• GSDMB acts as a downstream mediator of granzyme-mediated cell death, specifically activated through cleavage by granzyme A (GZMA). Related references: PMID:36899106, PMID:36991122, PMID:36991125
• Cleavage releases the N-terminal pore-forming domain, which binds to membrane inner leaflet lipids and homooligomerizes to form pores of 10–15 nm inner diameter. Related references: PMID:36899106, PMID:36991122, PMID:36991125
• Pore formation disrupts membrane integrity, triggering pyroptosis. Related references: PMID:36899106, PMID:36991122, PMID:36991125
• The full-length protein includes a C-terminal regulatory domain that autoinhibits the N-terminal pore-forming domain; the antibody targets both the full-length and C-terminal regions, making it suitable for studying GSDMB processing.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and standard SDS-PAGE to resolve GSDMB isoforms.
• Since the antibody recognizes the C-terminal region, it can detect both full-length and cleaved C-terminal fragments; confirm band sizes against the predicted molecular weights of 18, 45, 46, and 47 kDa.
• Validate antibody performance in human cell lines or tissues known to express GSDMB; include appropriate positive controls such as cells induced to undergo pyroptosis.
• For activation studies, treat cells with granzyme A or induce pyroptosis to monitor cleavage products and pore formation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-Gasdermin B polyclonal antibody, targeting a key effector of pyroptosis, exemplifies our capability to deliver high-quality reagents for cell death and inflammation research. Whether you need standard catalog products or customized rare-target sourcing, CamelBio serves as a reliable bridge from concept to clinic.