Anti-GAPVD1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14C86

Core Product Specifications and Parameters

Biological Background: GAPVD1 Function and Localization

• GAPVD1, also known as GAPex-5 or RAP6, is a GTPase-activating protein and VPS9 domain-containing protein that functions as both a GAP and a GEF, regulating small GTPases such as Rab5 and Ras.
• It plays a critical role in insulin-stimulated GLUT4 (SLC2A4) trafficking by acting as a GEF for RAB31, maintaining RAB31 in an active state to regulate GLUT4 storage vesicle cycling.
• GAPVD1 is involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent proteasomal degradation.
• It localizes to membrane and endosomal compartments, consistent with its function in endocytic trafficking.
• At the protein level, GAPVD1 is expressed in erythrocytes.
• The protein undergoes alternative splicing and is phosphorylated, as indicated by proteomics identification.
• Its predicted molecular weight is approximately 165 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-260), suggesting the antibody may recognize this epitope; consider verifying with deletion mutants or epitope mapping.
• For Western blot (WB), the predicted molecular weight is ~165 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be necessary to access endosome-associated GAPVD1.
• Validate antibody specificity using positive controls (e.g., cell lines expressing GAPVD1) and negative controls (e.g., knockdown or knockout models).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing solutions, from concept to clinic, for diagnostic manufacturers and research labs. This anti-GAPVD1 antibody supports studies in insulin signaling, endocytosis, and membrane trafficking. Beyond single antibodies, CamelBio can supply validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your assay development.