Anti-GAP43 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P17677

Core Product Specifications and Parameters

Biological Background: GAP43/Neuromodulin Function and Localization

• GAP43 (growth-associated protein 43), also known as neuromodulin, B-50, PP46, or GAP-43, is a key neuronal protein implicated in axonal pathfinding and regeneration.
• It is a major component of the motile growth cones that form the tips of elongating axons, where it drives the induction of axonal and dendritic filopodia.
• The protein is anchored to the cytoplasmic face of the cell membrane via palmitoylation, and it localizes to growth cone membranes, filopodium membranes, synapses, dendrites, axons, and the perikaryon.
• GAP43 is a phosphoprotein; phosphorylation at specific residues by protein kinase C is linked to calmodulin binding and release, modulating actin dynamics and growth cone motility.
• The gene undergoes alternative splicing, producing isoforms that may fine-tune its function in neuronal differentiation and synaptic plasticity.
• As a developmental protein and marker of neurogenesis, GAP43 expression is tightly regulated during development and is re-induced in regenerating or plastic adult neurons.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GAP43 protein (aa 1-238); the antibody may therefore recognize both phosphorylated and unphosphorylated forms, depending on the sample preparation.
• For western blot, a single band around 25 kDa (predicted molecular weight 24.8 kDa) is anticipated; validate detection in positive control lysates such as brain or neuronal cell lines.
• In immunofluorescence/ICC, expect punctate membrane staining concentrated at growth cones, axonal tips, and dendritic filopodia; co-stain with appropriate markers (e.g., Tau, MAP2, phalloidin) to confirm localization.
• Cross-reactivity with human, mouse, and rat is reported; for other species, sequence homology should be assessed and validation performed in the relevant sample system.
• Consider using knockout or knockdown controls to confirm antibody specificity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and neuroscience researchers as a comprehensive bridging partner for IVD raw-material procurement. Complementing this high-performance anti-GAP43 polyclonal antibody, our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support neurobiology and growth-cone studies. From rare-target sourcing to custom manufacturing, CamelBio streamlines your supply chain from concept to clinic.