Anti-GAP43 Monoclonal Antibody for WB, IF, ELISA - P17677

Core Product Specifications and Parameters

Biological Background: GAP43 Function and Localization

• GAP43, also known as neuromodulin, B-50, pp46, is a growth-associated protein primarily expressed in neurons, encoded by the GAP43 gene.
• It is a major component of motile growth cones at the tips of elongating axons and plays a critical role in axonal and dendritic filopodia induction.
• The protein is involved in nerve growth and neural development, contributing to differentiation, growth regulation, and neurogenesis.
• Subcellularly, GAP43 localizes to the cell membrane, growth cone membrane, synapse, filopodium membrane, perikaryon, dendrites, axons, and cytoplasm.
• GAP43 is a lipoprotein modified by palmitoylation and is also a phosphoprotein; it binds calmodulin and is subject to alternative splicing.
• The canonical isoform has a calculated molecular weight of approximately 24.8 kDa, with observed migration at around 25 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 139–238, suggesting the antibody likely recognizes a linear epitope; denaturing conditions (e.g., SDS-PAGE for WB) are expected to preserve the epitope.
• For Western blot, consider loading 20–30 µg of cell or tissue lysate; the predicted band is ~25 kDa, and a reduction step is typically used.
• For immunofluorescence (IF), both frozen (IF-F) and paraffin-embedded (IF-P) sections are supported; optimize fixation and permeabilization protocols and validate in positive and negative control samples.
• When using ELISA, ensure the coating antigen or capture format is compatible with the monoclonal antibody; titrate the antibody to determine the optimal working concentration for your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibody pairs and monoclonal antibodies like this GAP43 clone for nerve growth and neuroscience applications. Beyond this product, we offer one-stop sourcing for optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development and manufacturing.