Anti-GANAB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14697

Core Product Specifications and Parameters

Biological Background: GANAB Function and Localization

• Neutral alpha-glucosidase AB (GANAB), also known as glucosidase II subunit alpha, alpha-glucosidase 2, PKD3, GIIA, or GLUII, serves as the catalytic subunit of the glucosidase II complex.
• It catalyzes the sequential removal of the two innermost α-1,3-linked glucose residues from the Glc₂Man₉GlcNAc₂ oligosaccharide precursor of immature glycoproteins, a critical step in N-glycan processing. Related references: PMID:10929008
• GANAB is required for the maturation and proper localization of PKD1/polycystin-1 and PKD2/polycystin-2 to the cell surface and primary cilia, linking its activity to autosomal dominant polycystic kidney disease. Related references: PMID:27259053
• The protein contains a signal sequence, a glycoside hydrolase family 31 catalytic domain, and multiple disulfide bonds, consistent with its function in the endoplasmic reticulum.
• Subcellularly, GANAB localizes primarily to the endoplasmic reticulum and Golgi apparatus, and has also been detected in melanosomes.
• Tissue expression analysis shows GANAB in placenta, with isoform 1 and isoform 2 expressed in kidney and liver, reflecting their roles in secretory and absorptive tissues.
• Post-translational modifications include N-glycosylation and phosphorylation, which may regulate its activity or localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–260) of human GANAB (isoform NP_001265123.1); consider this when interpreting epitope accessibility in samples with alternative splicing or post-translational modifications.
• For western blotting, a band around 107 kDa is expected; validate in the relevant species (human, mouse, rat) and include appropriate controls to confirm specificity.
• In immunofluorescence or ICC, organelle markers (e.g., ER or Golgi) may be useful to corroborate the expected subcellular localization.
• ELISA performance may be influenced by protein folding and glycosylation of the target; titrate the antibody under assay-specific conditions.

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