Anti-gamma-Synuclein Monoclonal Antibody for WB - O76070

Core Product Specifications and Parameters

Biological Background: gamma-Synuclein Function and Localization

• Gamma-synuclein, also known as breast cancer-specific gene 1 protein (BCSG1) or synoretin (SR), is a member of the synuclein family, sharing homology with alpha- and beta-synuclein, and is encoded by the SNCG gene.
• It plays a role in neurofilament network integrity, potentially modulating axonal architecture during development and in adults, and increases the susceptibility of neurofilament-H to calcium-dependent proteases in vitro (By similarity).
• The protein may also function in modulating the keratin network in skin and can activate the MAPK and Elk-1 signal transduction pathway (By similarity).
• Gamma-synuclein is predominantly localized in the cytoplasm, particularly in the perinuclear region, and associates with the cytoskeleton at the centrosome and spindle microtubules.
• It is highly expressed in the brain, especially the substantia nigra, with additional expression in the corpus callosum, heart, skeletal muscle, ovary, testis, colon, and spleen, and weaker expression in the pancreas, kidney, and lung.
• The protein is subject to phosphorylation (as indicated by the Phosphoprotein keyword) and contains repeat regions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 48–127 of human gamma-synuclein (O76070), an internal region that lies within the protein's core domain, likely providing specific recognition of the target.
• For Western blot, consider using a standard dilution range suitable for rabbit monoclonal antibodies (e.g., 1:500–1:2000) and validate with a positive control such as brain or neuronal tissue lysates, where gamma-synuclein is highly expressed.
• In immunohistochemistry (IHC-P), antigen retrieval techniques may be necessary; test on sections of substantia nigra or corpus callosum as positive controls, and always include appropriate negative controls to confirm specificity.
• When performing ELISA, the recommended detection range depends on the specific assay format; optimize by titrating the antibody against immobilized peptide or recombinant protein to establish linearity and sensitivity.
• Because gamma-synuclein shares homology with alpha- and beta-synuclein, verify cross-reactivity patterns using cell lines or tissues expressing individual synuclein family members to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, from validated antibody pairs to optimized monoclonal and polyclonal antibodies, along with bulk ancillary reagents. For neuroscientists and researchers focusing on cytoskeletal organization and neurodegenerative diseases, CamelBio can source rare-target antibodies such as this anti-gamma-synuclein monoclonal antibody, ensuring a smooth transition from concept to clinic. Leverage CamelBio’s comprehensive supply network to accelerate your assay development and secure the raw materials you need.