Anti-GALT Polyclonal Antibody for WB, ELISA - P07902

Core Product Specifications and Parameters

Biological Background: GALT Function and Localization

• GALT (Galactose-1-phosphate uridylyltransferase) is a key enzyme in the Leloir pathway of galactose metabolism, converting galactose-1-phosphate and UDP-glucose to UDP-galactose and glucose-1-phosphate.
• The enzyme belongs to the nucleotidyltransferase family and requires zinc as a cofactor for catalytic activity.
• Alternative splicing of GALT mRNA produces multiple isoforms, which may have distinct tissue-specific roles or regulatory functions.
• Mutations in the GALT gene are associated with classical galactosemia, a metabolic disorder characterized by symptoms including cataracts, liver damage, and intellectual disability if untreated.
• The protein has a molecular weight of approximately 43 kDa and its three-dimensional structure has been determined, providing insights into its catalytic mechanism.
• GALT is recognized by proteomics identification methods and serves as a reference proteome component for metabolic studies.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human GALT protein (aa 1-379); the antibody may detect full-length and potentially truncated isoforms. Validate specificity in your target sample.
• For Western blotting, a band at ~43 kDa is expected in human, mouse, and rat lysates. Optimize loading and detection conditions to minimize background.
• For ELISA applications, the polyclonal nature offers high avidity. Consider checkerboard titrations to determine optimal coating and detection antibody concentrations.
• Storage and handling: ship on ice bag; aliquot and store at -20°C or -80°C upon receipt for long-term stability. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop platform providing IVD raw materials, from validated antibodies and recombinant proteins to bulk ancillary reagents. This anti-GALT polyclonal antibody supports research in galactose metabolism and related disorders, and can be integrated into diagnostic assay development. Our expert team also assists with rare-target sourcing and customized production solutions to streamline your project from concept to clinic.