Anti-GALNT7 Polyclonal Antibody for WB, IHC-P, ELISA - Q86SF2

Core Product Specifications and Parameters

Biological Background: GALNT7 Function and Localization

• GALNT7 (N-acetylgalactosaminyltransferase 7), also known as GalNAc-T7 or GALNAC-T7, is a member of the glycosyltransferase family that catalyzes the transfer of N-acetyl-D-galactosamine (GalNAc) to serine/threonine residues in proteins, initiating mucin-type O-linked glycosylation.
• Unlike other GalNAc transferases, GALNT7 primarily functions as a glycopeptide transferase, requiring a pre-existing GalNAc residue on the acceptor peptide to add additional GalNAc moieties, playing a role in extending O-glycan chains rather than initiating them.
• The protein contains a lectin domain that binds GalNAc, contributing to its substrate specificity and processive catalytic mechanism; it also requires manganese as a cofactor.
• Encoded by the GALNT7 gene (Gene ID: 51809) in humans, GALNT7 is widely expressed, with higher levels observed in uterus, retina, kidney, small intestine, omentum, stomach, and central nervous system.
• Subcellularly, GALNT7 localizes to the Golgi apparatus membrane via a single-pass transmembrane domain, consistent with its role in post-translational modification within the secretory pathway.
• Key structural features include a signal-anchor sequence, disulfide bonds, and a GT-A fold catalytic domain typical of glycosyltransferases, as revealed by its 3D structure (UniProt keyword: 3D-structure).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30-130 of human GALNT7, a region that may include part of the catalytic domain; consider verifying specificity with appropriate controls, such as knockout cell lysates or peptide blocking.
• For Western blotting, the predicted molecular weight is approximately 75 kDa; always include pre-stained protein markers and confirm band size in your sample type.
• In immunohistochemistry (IHC-P), GALNT7 is expressed in various tissues including uterus, kidney, and stomach; optimize antigen retrieval and primary antibody dilution for each tissue to minimize non-specific staining.
• For ELISA, the antibody can be used for antigen detection; however, since the target is a Golgi membrane protein, use detergent-solubilized lysates or recombinant protein standards to ensure proper antigen presentation.
• As a polyclonal antibody, lot-to-lot variability is possible; re-validate the antibody in your assay when switching to a new batch.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your trusted strategic partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and handle rare target raw-material sourcing for glycobiology and cancer research. Explore our catalog to accelerate your assay development with high-quality GALNT7 antibodies and associated IVD reagents.