Anti-GALNT6 Polyclonal Antibody for WB, ELISA - Q8NCL4

Core Product Specifications and Parameters

Biological Background: GALNT6 Function and Localization

• Acts as a polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6), initiating O-linked glycosylation by transferring N-acetyl-D-galactosamine to serine or threonine residues.
  Related references:
  PMID:10464263
  PMID:31932717
• Recognizes and glycosylates substrates such as MUC1A, MUC2, EA2, fibronectin peptides, and FGF23; contributes to oncofetal fibronectin biosynthesis.
  Related references:
  PMID:10464263
  PMID:31932717
• Resides in the Golgi apparatus membrane, consistent with its role in post-translational modification in the secretory pathway.
• Exhibits tissue-specific expression, with highest levels in placenta and trachea; weak expression in brain, pancreas, and fibroblasts.
• Contains a signal-anchor, a transmembrane domain, and a lectin domain; classified under glycosyltransferase family, requiring manganese as a cofactor.
• Annotated with keywords including disulfide bond, glycoprotein, metal-binding, and transferase activity, reflecting its structural and functional attributes.

Experimental Guidance and Technical Tips

• For Western blot, use reducing SDS‑PAGE; the expected band is at ~71 kDa; consider loading 20–30 µg of lysate from placenta or trachea tissue as a positive control.
• For ELISA, the antibody can serve as a detection reagent; validate performance with recombinant GALNT6 protein or relevant peptide substrates in your assay system.
• Since the immunogen corresponds to amino acids 30–140 (N-terminal region), the antibody likely recognizes epitopes within the catalytic stem domain; verify specificity using knockout or knockdown models.
• Optimize antibody dilution empirically; typical starting ranges are 1:500–1:2000 for WB and 1:1000–1:5000 for ELISA, but adjust based on your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw-material sourcing, offering this anti-GALNT6 polyclonal antibody alongside a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. We support manufacturers and researchers in glycosylation-focused diagnostic development, from early feasibility to large‑scale production. Contact us for custom bulk orders, matched antibody pairs, or assistance with rare target procurement.