Anti-GALNT2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q10471

Core Product Specifications and Parameters

Biological Background: GALNT2 Function and Localization

• GALNT2 encodes polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2), a Golgi-resident enzyme that initiates O-linked glycosylation by transferring N-acetyl-D-galactosamine (GalNAc) to serine or threonine residues of target proteins.
• It exhibits broad substrate specificity, glycosylating peptides (EA2), mucins (Muc5AC, Muc1a, Muc1b), and proteins such as apolipoprotein C-III (APOC-III), angiopoietin-like 3 (ANGPTL3), and phospholipid transfer protein (PLTP). Plays a role in regulating high-density lipoprotein cholesterol (HDL-C) metabolism (PubMed:27508872, PubMed:32293671). Related references: PMID:27508872 PMID:32293671
• It glycosylates the hinge region of immunoglobulin A1 (IgA1), potentially influencing IgA nephropathy pathogenesis.
• Localizes to the Golgi stack membrane and is also secreted; detectable in urine at the protein level (PubMed:37453717). Related references: PMID:37453717
• Widely expressed across human tissues, reflecting its fundamental role in glycobiology.
• Features a glycosyltransferase domain, a lectin domain, and a single-pass transmembrane helix; requires manganese as a cofactor.
• Mutations in GALNT2 are linked to congenital disorder of glycosylation type IIt (CDG2T). The protein undergoes alternative splicing and phosphorylation.
• Catalytic activity and substrate selection are influenced by its lectin domain, which binds GalNAc-modified substrates.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 442-571) of human GALNT2, which includes part of the lectin domain. This region is conserved among human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot (WB), a band around 65 kDa (predicted MW) is expected; consider using positive controls from cell lines expressing GALNT2.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; cytoplasmic/Golgi-like staining patterns should be anticipated.
• For immunofluorescence (IF/ICC), permeabilization is required to detect intracellular Golgi localization.
• For ELISA, the antibody can be used as a capture/detection reagent; validate pairing with appropriate standards.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers by supplying a comprehensive portfolio of IVD antibodies, including this anti-GALNT2 polyclonal antibody for glycobiology and metabolic disease research. Our sourcing capabilities span validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized procurement of rare-target raw materials, streamlining your path from concept to clinic.