Anti-GALNS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P34059

Core Product Specifications and Parameters

Biological Background: GALNS Function and Localization

• GALNS encodes N-acetylgalactosamine-6-sulfatase, a lysosomal sulfatase responsible for hydrolyzing sulfate groups from glycosaminoglycans, specifically keratan sulfate and chondroitin-6-sulfate.
• This enzyme plays a crucial role in the stepwise degradation of glycosaminoglycans within the lysosome, facilitating their recycling.
• Mutations in the GALNS gene lead to mucopolysaccharidosis IVA (Morquio syndrome A), an autosomal recessive lysosomal storage disorder characterized by skeletal abnormalities and dwarfism.
• The protein is synthesized as a precursor with an N-terminal signal peptide, undergoes glycosylation, and contains conserved disulfide bonds and calcium-binding sites that are essential for its structure and catalytic activity.
• Subcellularly, GALNS localizes exclusively to the lysosomal lumen where it functions optimally at acidic pH.
• Alternative names for this enzyme include chondroitinsulfatase (chondroitinase), galactose-6-sulfate sulfatase (GalN6S), and N-acetylgalactosamine-6-sulfate sulfatase (GALNAC6S).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 353-522 of human GALNS; consider this epitope location when validating the antibody in your specific experimental context.
• For Western blot (WB), the predicted molecular weight is approximately 58 kDa; run appropriate molecular weight markers and consider loading controls.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to ensure accessibility to the lysosomal antigen; a mild permeabilization step is recommended.
• The antibody is validated for human, mouse, and rat reactivity; due to high sequence homology, it may also recognize GALNS from other species, but this should be verified experimentally.
• As a polyclonal antibody, lot-to-lot variability may occur; it is advisable to titrate the antibody and validate its performance in your specific assay and sample type.
• For ELISA, consider pairing with a suitable detection antibody and optimizing coating conditions to achieve the desired sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, supplying high-quality reagents from concept to clinic. This anti-GALNS polyclonal antibody is an excellent tool for research into lysosomal storage disorders, particularly mucopolysaccharidosis IVA, and supports assay development in diagnostic and academic settings. With capabilities in providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, CamelBio streamlines your procurement process, ensuring you have the right materials for every stage of development.