Anti-GALK1 Rabbit Polyclonal Antibody for WB, ELISA - P51570

Core Product Specifications and Parameters

Biological Background: GALK1 Function and Localization

• GALK1 (galactokinase 1) catalyzes the ATP-dependent phosphorylation of alpha-D-galactose, the first committed step in the Leloir pathway of galactose metabolism.
• As a member of the GHMP kinase family, it possesses conserved nucleotide-binding and substrate-recognition motifs critical for its kinase activity.
• The protein is classified as a transferase and a phosphoprotein, with evidence of phosphorylation regulating its function.
• Mutations in GALK1 cause galactokinase deficiency, a disorder linked to cataract formation, highlighting its clinical relevance as a disease-associated enzyme.
• The 3D structure of GALK1 has been solved, providing insights into its catalytic mechanism and substrate binding.
• Direct protein sequencing and high-throughput proteomics have confirmed its expression across human tissues, placing it in the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (residues 1–120) of human GALK1; verify performance in your target tissue or cell line.
• For Western blotting, expect a band near 42 kDa and optimize antibody dilution using positive controls such as liver or HEK293 lysates.
• In ELISA, consider using this polyclonal as a capture or detection reagent after appropriate validation and pairing.
• Cross-reactivity with mouse GALK1 makes it suitable for rodent model studies; however, confirm species specificity in your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a broad range of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-GALK1 antibody serves research in galactose metabolism and kinase pathways. For custom sourcing or large-volume requirements, CamelBio provides end-to-end support from concept to clinic.