Anti-Galectin 4/LGALS4 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P56470

Core Product Specifications and Parameters

Biological Background: Galectin-4 Function and Localization

• Human Galectin-4 (LGALS4 gene) encodes a 35.9 kDa lactose-binding lectin, also known as L-36 lactose-binding protein (L36LBP) and antigen NY-CO-27.
• Galectin-4 binds lactose and a related range of sugars, functioning as a carbohydrate recognition protein in cell-cell and cell-matrix interactions.
• It may participate in the assembly of adherens junctions, suggesting a role in epithelial cell adhesion and polarity.
• The protein contains conserved carbohydrate-recognition domains (CRDs) with structural repeats, characteristic of tandem-repeat galectins.
• Galectin-4 is regulated by phosphorylation, a common post-translational modification that may modulate its lectin activity and localization.

Experimental Guidance and Technical Tips

• For WB analysis, test both native and denaturing conditions, as the polyclonal antibody may recognize conformational and linear epitopes from the N-terminal immunogen (aa1-100).
• In IF-P experiments, optimize permeabilization and fixation protocols to preserve antigen accessibility; consider antigen retrieval if necessary.
• When performing ELISA, determine optimal coating concentrations of the recombinant protein and serum titration for the detection antibody.
• The antibody shows cross-reactivity with mouse and rat, enabling comparative studies across species; validate in the appropriate samples.
• The immunogen is an N-terminal fragment (aa1-100); hence, the antibody may preferentially detect the N-terminal region and may not be suitable for C-terminal tag-based detection without validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to provide a comprehensive portfolio of IVD raw materials, including validated antibodies and ancillary reagents. For galactose-binding lectin research, we offer this anti-Galectin-4 polyclonal antibody alongside other related reagents to support your assay development and biomarker exploration needs. Our dedicated sourcing service ensures efficient access to rare targets and bulk orders, facilitating seamless translation from concept to clinic.