Anti-Galectin 4/LGALS4 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P56470

Core Product Specifications and Parameters

Biological Background: Galectin 4/LGALS4 Function and Localization

• Galectin-4, encoded by the LGALS4 gene, is a member of the galectin family of carbohydrate-binding proteins, also known as antigen NY-CO-27, L-36 lactose-binding protein (L36LBP), or lactose-binding lectin 4.
• It specifically binds to beta-galactoside sugars, such as lactose, and a related range of sugars.
• It may play a role in the assembly of adherens junctions, suggesting involvement in cell-cell adhesion and epithelial integrity.
• Galectin-4 contains two carbohydrate recognition domains (CRDs) arranged in tandem, as indicated by the 'Repeat' keyword in its UniProt annotation.
• It is a phosphoprotein, and its function may be regulated by phosphorylation.
• The calculated molecular weight is approximately 35.9 kDa, though it migrates at about 36 kDa in SDS-PAGE.
• As a galectin, it is likely found in the cytoplasm and can be secreted to the extracellular space; its involvement in adherens junctions suggests plasma membrane association.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100) of Galectin-4; the antibody likely recognizes an epitope within this segment. Consider using it to detect full-length Galectin-4 and N-terminal fragments.
• For Western blot, expect a band around 36 kDa. Validate specificity with appropriate controls (e.g., knockdown lysates or peptide blocking).
• For IHC-P and IF-P, optimize fixation and antigen retrieval carefully, as galectins are soluble and may require gentle tissue processing.
• Observed cross-reactivity with mouse and rat should be validated in your sample system to confirm reactivity and absence of species-specific non‑specific bands.
• In ELISA, this antibody may serve as a capture or detection reagent; consider paired antibody testing for optimal assay design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high‑quality IVD raw materials. For galectin-4/LGALS4‑related studies, we offer validated antibody pairs, optimized monoclonal antibodies like this clone, and bulk ancillary reagents. Our sourcing network also supports rare targets, helping you secure the critical raw materials needed from concept to clinic.